minianalysis program v 6.0.7 Search Results


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grpcre  (Jackson Laboratory)
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Figure 7. CB2R agonist treatments suppress excitatory synaptic transmission of spinal itch-related neurons (A and B) Representative traces and quantification of sEPSCs in spinal GRP neurons from <t>GRPCre;Ai9.</t> n = 8–11 neurons/group. (C and D) Representative traces and quantification of sEPSCs in spinal GRPR neurons <t>from</t> <t>GRPRCreERT2;Ai9</t> mice. n = 13–18 neurons/group. (E–H) Representative traces and quantification of sEPSCs in GRP neurons and GRPR neurons after minocycline perfusion. n = 15 GRP neurons and 10 GRPR neurons. Paired t test. (I) Schematic diagram for injection of rAAV into the bilateral PBN of Vglut2Cre mice. Top right: representative image of mCherry in the projection neuron terminals in the PBN. Scale bars: 100 mm. Bottom right: representative image of mCherry in the Vgult2+ projection neuron in the SDH. Scale bars: 10 mm. (J and K) Representative traces and quantification of AP firings in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. Two-way ANOVA Sidak’s multiple comparisons. n = 13–14 neurons/group. (L and M) Representative traces and quantification of sEPSCs in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. n = 11–12 neurons/group. (B, D, and M) One-way ANOVA with Tukey’s multiple comparisons. All data are mean ± SEM. Veh., vehicle; BL, baseline; Mino., minocycline.
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mouse  (Jackson Laboratory)
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Jackson Laboratory mouse
Figure 7. CB2R agonist treatments suppress excitatory synaptic transmission of spinal itch-related neurons (A and B) Representative traces and quantification of sEPSCs in spinal GRP neurons from <t>GRPCre;Ai9.</t> n = 8–11 neurons/group. (C and D) Representative traces and quantification of sEPSCs in spinal GRPR neurons <t>from</t> <t>GRPRCreERT2;Ai9</t> mice. n = 13–18 neurons/group. (E–H) Representative traces and quantification of sEPSCs in GRP neurons and GRPR neurons after minocycline perfusion. n = 15 GRP neurons and 10 GRPR neurons. Paired t test. (I) Schematic diagram for injection of rAAV into the bilateral PBN of Vglut2Cre mice. Top right: representative image of mCherry in the projection neuron terminals in the PBN. Scale bars: 100 mm. Bottom right: representative image of mCherry in the Vgult2+ projection neuron in the SDH. Scale bars: 10 mm. (J and K) Representative traces and quantification of AP firings in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. Two-way ANOVA Sidak’s multiple comparisons. n = 13–14 neurons/group. (L and M) Representative traces and quantification of sEPSCs in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. n = 11–12 neurons/group. (B, D, and M) One-way ANOVA with Tukey’s multiple comparisons. All data are mean ± SEM. Veh., vehicle; BL, baseline; Mino., minocycline.
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Image Search Results


Figure 7. CB2R agonist treatments suppress excitatory synaptic transmission of spinal itch-related neurons (A and B) Representative traces and quantification of sEPSCs in spinal GRP neurons from GRPCre;Ai9. n = 8–11 neurons/group. (C and D) Representative traces and quantification of sEPSCs in spinal GRPR neurons from GRPRCreERT2;Ai9 mice. n = 13–18 neurons/group. (E–H) Representative traces and quantification of sEPSCs in GRP neurons and GRPR neurons after minocycline perfusion. n = 15 GRP neurons and 10 GRPR neurons. Paired t test. (I) Schematic diagram for injection of rAAV into the bilateral PBN of Vglut2Cre mice. Top right: representative image of mCherry in the projection neuron terminals in the PBN. Scale bars: 100 mm. Bottom right: representative image of mCherry in the Vgult2+ projection neuron in the SDH. Scale bars: 10 mm. (J and K) Representative traces and quantification of AP firings in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. Two-way ANOVA Sidak’s multiple comparisons. n = 13–14 neurons/group. (L and M) Representative traces and quantification of sEPSCs in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. n = 11–12 neurons/group. (B, D, and M) One-way ANOVA with Tukey’s multiple comparisons. All data are mean ± SEM. Veh., vehicle; BL, baseline; Mino., minocycline.

Journal: Cell reports

Article Title: Cannabinoid CB 2 receptor controls chronic itch by regulating spinal microglial activation and synaptic transmission.

doi: 10.1016/j.celrep.2025.115559

Figure Lengend Snippet: Figure 7. CB2R agonist treatments suppress excitatory synaptic transmission of spinal itch-related neurons (A and B) Representative traces and quantification of sEPSCs in spinal GRP neurons from GRPCre;Ai9. n = 8–11 neurons/group. (C and D) Representative traces and quantification of sEPSCs in spinal GRPR neurons from GRPRCreERT2;Ai9 mice. n = 13–18 neurons/group. (E–H) Representative traces and quantification of sEPSCs in GRP neurons and GRPR neurons after minocycline perfusion. n = 15 GRP neurons and 10 GRPR neurons. Paired t test. (I) Schematic diagram for injection of rAAV into the bilateral PBN of Vglut2Cre mice. Top right: representative image of mCherry in the projection neuron terminals in the PBN. Scale bars: 100 mm. Bottom right: representative image of mCherry in the Vgult2+ projection neuron in the SDH. Scale bars: 10 mm. (J and K) Representative traces and quantification of AP firings in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. Two-way ANOVA Sidak’s multiple comparisons. n = 13–14 neurons/group. (L and M) Representative traces and quantification of sEPSCs in spinal lamina I projection neurons from Vglut2Cre;rAAV mice. n = 11–12 neurons/group. (B, D, and M) One-way ANOVA with Tukey’s multiple comparisons. All data are mean ± SEM. Veh., vehicle; BL, baseline; Mino., minocycline.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GEXSCOPE Single Cell RNA Library Kit Singleron Biotechnologies Cat# 5180011 RNAsimple Total RNA Kit TianGen Cat# DP419 PrimeScriptTM RT regent Kit with gDNA Eraser Takara Cat# RR047A TB Green premix Ex Taq Takara Cat# RR820A Enhanced chemiluminescence reagents New Cell Molecular Biotechnology Co.,Ltd Cat# P10200 BCA Protein Assay Kit Beyotime Cat# P0012S Deposited data Raw and analyzed data This paper GEO: GSE242768 Experimental models: Cell lines Mouse primary spinal cord microglia This paper N/A BV2 cell Procell Life Science & Technology Co.,Ltd Cat# CL-0493A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX: 000664 Mouse: CB2R / : B6.129P2-Cnr2tm1Dgen/J The Jackson Laboratory RRID:IMSR_JAX: 005786 Mouse: Tmem119CreERT2: C57BL/6Tmem119em1(cre/ERT2)Gfng/J The Jackson Laboratory RRID:IMSR_JAX: 031820 Mouse: R26tdTomato (Ai9): B6.129S6-Gt (ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX: 007905 Mouse: R26iDTR: C57BL/6-Gt(ROSA) 26Sortm1 (HBEGF)Awai/J The Jackson Laboratory RRID:IMSR_JAX: 007900 Mouse: R26LSL-hM4Di: B6.129-Gt(ROSA) 26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J The Jackson Laboratory RRID:IMSR_JAX: 026219 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID:IMSR_JAX: 028863 Mouse: R26LSL-hM3Dq: B6N; 129-Tg (CAG-CHRM3*,-mCitrine)1Ute/J The Jackson Laboratory RRID:IMSR_JAX: 026220 Mouse: C57BL/6JGpt-Cnr2em1Cflox/Gpt GemPharmatech Strain NO. T052045 Mouse: CX3CR1GFP/GFP The Jackson Laboratory RRID:IMSR_JAX: 005582 Mouse: GRPCre The Jackson Laboratory RRID:IMSR_JAX: 033174 Mouse: GRPRCreERT2 Sun Yangang Lab PMID: 28818946 Oligonucleotides Primers for RT-qPCR, see Table S2 This paper N/A Software and algorithms Prism GraphPad 9.0 GraphPad Software http://www.graphpad.com Fiji (ImageJ) NIH https://imagej.nih.gov/ij/ Zeiss ZEN 3.4 (blue edition) Zeiss www.zeiss.com/microscopy Aperio ImageScope 9.0 Aperio Technologies http://www.aperio.com Mini Analysis Program version 6.0.7 Synaptosoft Inc. http://www.synaptosoft.com/MiniAnalysis/ HEKA Patchmaster software HEKA https://www.heka.com/ qPCRsoft v.4.1 Analytik Jena http://www.analytik-jena.com Imaris v9.3.1 Bitplane http://imaris.oxinst.com BioRender BioRender https://www.biorender.com/ Adobe Photoshop Adobe http://adobe.com FlowJo v10.8.1.

Techniques: Transmission Assay, Injection